No more weekly updates.... Progress is soooo slow that there is nothing talking about. Well... there is a lot of negative stuff. Take today for example.
Today is a public holiday. Almost nobody comes to work. I am supposed to several straightforward things:
1. Prepare biochemical
2. Treat cells
3. Plate cells for tomorrow
4. Aliquot FBS
5. Assay organism
6. Test biochemical standard
Item 1: Ok.
Item 2: Ok.
Item 3: The pipette has no marker below 8ml. So I assume it's a 10ml pipette as most of the pipettes in these
cylinders are 10ml pipettes. I plated the cells. Then I found that I had a lot of excess -- which means
the pipette is actually a 11ml pipette! Ok. Have to scrap experiment and wait for cells to grow.
Item 4: Ok.
Item 5: After the horror of item 3, I was disheartened. In addition, I need to pH the medium before I can
use. The assay will take ~2.5 hours. Anyway, I think I'll go do Item 6 first.
Item 6: Embarrassing failure. Somehow, the pipetting was way off. Yesterday I got R2 of above 0.999.
(I usually get R2 of above 0.99) Today, I do not even need to plot the graph, I know it is "gone
case". This proves that I should not do Item 5. Time to pack up and go home.
Lesson learnt: Try to avoid working on public holidays if I can.
HOWEVER, work is still work. No matter what comes, I have to complete it. This is a promise to myself and to many people.
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