Project Final Fantasy Update: Week 10.5

Week 10.5. Reconstituted all 3 drugs (unsure if they are contaminated in the process) and assay kit. Performed plating test for 24-well plate. Subcultured 5 flasks of cells. Made new culture reagents. I am behind time. Why? Because I have not been careful enough - strange things appeared in my flasks, despite the precautions I have taken since the last incidence. I must learn not to take things for granted. Biochemical C staining came out alright. That is the only good thing that came out. Organism assay need to redo certain parts. Sometimes, I hate myself for not being able to perform these simple tasks well.

On social front, I think my lab is a super close group. We got together for farewell dinner to 2 of our colleagues.We have lunch together, tea break together, breakfast together. Very, very nice. I really really want to earn my keep and prove that I can truly be one of them. Also, there is a new project that requires me to work with new people. I have seen only 1 of them (out of 4). Hopefully we can work well together and produce good results.

Note to self: It does not matter what others are doing. As long as I have done all that I can and all that is right, I am progressing. It is my progess that counts. The "enemy" is me. As long as I have surpassed myself, I would have surpassed most of the others. Real GN drive will prevail over false drives.

Before Official Start of Final Operation...

With 3 preparatory years exhausted, the time has come for the real challenge. Challenge in the real environment. Yes. I can memorize. I can think (though not as well as I liked). I can endure (though not as determined as before, but it is improving). However, it is the critical-on-the-spot thinking that I lack and the ability to plan around schedules that I am left wanting. Oh yes. I forgot to mention the perpetual trouble of being careless (or absent-minded). Through this challenge, I hope to seal these gaps. Only then, can I compete with the top people.

It is time to grow. Grow faster than anyone else!

Note to self: Knowing when to rest is important also. Remember: Haste makes waste.

Warning Signs

While compiling data, a few "problems" cropped up.

1. According to my assay, the amount of free biochemical  is actually more than that of total biochemical. (Problem with my data?)
2. Cell confluency used in assay is slightly less than that of that for Organism infected assay. (problem with correlating the data?)

In any case, we have ran out of the assay kit. When the kit comes, I think I will need to do a new repeat to confirm the data. Looks like I have not done so much work after all.